In callus culture from leaf explants, four compounds from groups of alcohol, acetic acid and organosilicon were identified, whereas in callus culture from stem explants were identified eight compounds from aldehydes, esters, alkanes, and carboxylic acids group. The results showed that various types of secondary metabolites contained in shoot culture, callus and field plants of C. The secondary metabolite compouds were analized and identified by GC-MS ( Gas Chromatography-Mass Spectrometry). For shoot culture, single nodule explants with one leaf were planted on MS media with the addition of 1 ppm BAP. morifolium plantlet stems and leaves respectively on MS medium with an addition of 3 ppm 2,4-D + 2 ppm kinetin and 4 ppm 2,4-D. The research method was exploration in the laboratory to analyze and compare the content of secondary metabolite from shoot culture, callus and field plants of C. The study aimed to determine the types of secondary metabolites that contained in shoot culture, callus and field plants of C. Tissue culture can be an alternative to enhance the production of certain secondary metabolite. The chrysanthemum plant ( Chrysanthemum morifolium Ramat.) contains many secondary metabolites such as flavonoids and various volatile compounds that can be utilized as drugs.
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